The Bovine Cornea Opacity and Permeability Test
نویسنده
چکیده
An in vitro test for the development of corneal opacity in response ocular irritants was first proposed by Muir (1984). This test required the clamping of a bovine cornea between two chambers filled with physiologic saline and measuring the transmittance of white light through the cornea using the voltage output of a photocell. This was followed by exposure of the epithelial surface of the cornea to a surfactant and subsequent measurement of light transmittance, with a decrease in transmittance due to development of corneal opacity interpreted as a toxic effect of the surfactant on the cornea.
منابع مشابه
EXPERT PANEL REPORT: Evaluation of the Current Validation Status of In Vitro Test Methods for Identifying Ocular Corrosives and Severe Irritants (March 2005) III. Bovine Corneal Opacity and Permeability Test Method
Page 57 § 1.1.2.: it is mentioned that some materials can cause serious corneal injury without appearing to change opacity or permeability immediately (e.g. mustard gas). Is this a specific observation for mustard gas or are other examples (agents) known which can induce damage without changes in opacity and/or permeability? Injury (cell death) without any change in opacity and/or permeability ...
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